Certificate of Analysis and Data Sheet

Source:
Synthetic

Catalog No.
HOR-262

Background:

Calcitonin (CT) is a peptide hormone produced by the parafollicular cells of the thyroid gland in mammals and by the ultimobranchial gland of birds and fish. Salmon calcitonin (sCT), which is more potent and longer lasting than human CT, has been used widely for the treatment of osteoporosis, paget's disease, hypercalcemic shock and chronic pain in terminal cancer patients. sCT is one of the many bioactive peptides that require C-terminal amidation for full biological activity.

Description :

Calcitonin Acetate (Salmon) is a synthetic polypeptide of 32 amino acids in the same linear sequence that is found in clacitonin of salmon origin.
MW=3432

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Solubility:

The lyophilized Calcitonin is very soluble in water and most aqueous buffers below and above the isoelectric point.

Stability:

Lyophilized Calcitonin although stable at room temperature for 3 weeks, should be stored desiccated below -18蚓. Upon reconstitution Calcitonin should be stored at 4蚓 between 2-7 days and for future use below -18蚓.
Please avoid freeze-thaw cycles.

Purity:

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence:

Cys-Ser-Asn-Leu-Ser-Thr-Cys-Val-Leu-Gly-Lys-Leu-Ser-Gln-Glu-Leu-His-Lys-Leu-Gln-Thr-Tyr-Pro-Arg-Thr-Asn-Thr-Gly-Ser-Gly-Thr-Pro-NH2

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Calcitonin.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm .
2. Analysis by RP-HPLC.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Protein synonyms/aliases:

Calcitonin-3.

Protein Family:

Belongs to the calcitonin family.

Protein Domains:

Domains:
•IPR001693   Calcitonin-like

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Passive and iontophoretic controlled delivery of salmon calcitonin through artificial membranes.
Curr Drug Deliv 2004 Apr;1(2):137-43
2. Hypercalcemia and status epilepticus relates to salmon calcitonin administration in breast cancer.
Breast 2005 Oct;14(5):399-402
3. The effects of salmon calcitonin-induced hypocalcemia on bone metabolism in ovariectomized rats.
J Bone Miner Metab 2005;23(5):359-65
4. Effects of salmon calcitonin on trabecular microarchitecture as determined by magnetic resonance imaging: results from the QUEST study.
J Bone Miner Res 2005 Sep;20(9):1548-61
5. The effects of synthetic salmon calcitonin on thyroid C and follicular cells in adult female rats.
Folia Histochem Cytobiol 2005;43(2):103-8
6. Clinical outcome of salmon calcitonin nasal spray treatment in postmenopausal women after total hip arthroplasty.
Gerontology 2005 Jul-Aug;51(4):242-52

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中華民國95年06月06日更新