Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-345

Background:

Troponin Complex is a heteromeric protein playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, Troponin I , Troponin T and Troponin C. Each subunit is responsible for part of Troponin Complex function. E.g. Troponin I inhibits ATP-ase activity of acto-myosin. Troponin T and Troponin I are presented in cardiac muscles in different forms than in skeletal muscles.

Description:

Recombinant scIC produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 45kDa.

Physical Appearance:

Sterile Filtered colourless liquid formualtion

Formulation:

0.5 mg/ml glycerol

Stability:

Recombinant Single Chain Cardiac Troponin I-C Complex is stable at 10蚓 between 2-7 days ,however, should be stored desiccated below -18蚓.
Please avoid freeze-thaw cycles.

Purity:

Greater than 85.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Single Chain Troponin I-C Complex .

Applications:

1. Immunoassays
2. Western blot

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Recombinant single chain cardiac troponin I-C polypeptides: superior calibration and control materials for cardiac troponin I immunoassays.
Clin Lab 2001;47(1-2):19-27