Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-346

Background:

Troponin Complex is a heteromeric protein playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, Troponin I , Troponin T and Troponin C. Each subunit is responsible for part of Troponin Complex function. E.g. Troponin I inhibits ATP-ase activity of acto-myosin. Troponin T and Troponin I are presented in cardiac muscles in different forms than in skeletal muscles.

Physical Appearance:

Sterile Filtered colourless liquid formualtion.

Formulation:

Contains 50% glycerol.

Stability:

Recombinant IC2 although stable at 10⁰C for 7 days, should be stored desiccated below -18⁰C.
Please avoid freeze-thaw cycles.

Purity:

Greater than 85.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant Single Chain IC2.

Applications:

Immunoassays
Western blot

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Recombinant single chain cardiac troponin I-C polypeptides: superior calibration and control materials for cardiac troponin I immunoassays.
Clin Lab 2001;47(1-2):19-27