Certificate of Analysis and Data Sheet

Source:
Baculovirus

Catalog No.
CYT-304

Background:

CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MHC (Major Histocompatibility Complex) Class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as HIV receptors, binding directly to the envelope protein gp120 on HIV. Thymocytes which are CD4 (+)and CD8 ( - )produce factors such as IL-4 , IL-5 ,IL-10 and IFN-gamma

Description :

Recombinant Human sCD4 produced in Baculovirus is a single, glycosylated polypeptide chain containing 365 amino acids and having a non glycosilated molecular mass of 40,000 Dalton. As a result of glycosilation the sCD4 migrates as a 46,000 dalton protein on standard SDS-PAGE. Detected By Western Imunno Blots anti cd4 monoclonal antibody. Recombinant soluble CD4 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered colorless liquid formulation.

Formulation:

Recombinant Human Soluble CD-4 solution contains 10mM sodium phosphate buffer pH-7.2, 300mM sodium chloride and 0.01% tween.

Stability:

rHusCD4 although stable at 14蚓 for 1 week, should be stored desiccated below -18蚓. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec旧 Recombinant Human Soluble CD-4 is fully biologically active when compared to standard. Immune reactive in ELISA using monoclonal anti CD4 in gpl2O capture ELISA assays.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of soluble CD4.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of CD4 as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CD4

Protein synonyms/aliases:

T-cell surface glycoprotein CD4 precursor (T-cell surface antigenT4/Leu-3).

Protein Family:

Protein Domains:

Domains:
•IPR013151   Immunoglobulin
•IPR007110   Immunoglobulin-like
•IPR008424   T-cell surface antigen CD2

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Identification of two N-linked glycosylation sites within the core of the simian immunodeficiency virus glycoprotein whose removal enhances sensitivity to soluble CD4.
J Virol 2005 Oct;79(19):12575-83
2. [Concentration of soluble CD4, CD8, CD25 receptors as well IFN-gamma and IL-4 released by lymphocyte of chronic Lyme patients cultured with 3 genotypes of Borrelia burgdorferi]
Pol Merkuriusz Lek 2004 May;16(95):447-50
3. DNA vaccines expressing soluble CD4-envelope proteins fused to C3d elicit cross-reactive neutralizing antibodies to HIV-1.
Virology 2004 Oct 25;328(2):292-300
4. [Soluble CD4 antigens in the cerebrospinal fluid as a marker of lymphocytic infiltration of the central nervous system]
Vojnosanit Pregl 2004 May-Jun;61(3):247-53
5. Detection of IgA inhibiting the interaction between gp120 and soluble CD4 receptor in serum and saliva of HIV-1-infected patients.
AIDS 2004 Jan 2;18(1):37-43
6. The prolonged culture of human immunodeficiency virus type 1 in primary lymphocytes increases its sensitivity to neutralization by soluble CD4.
Virology 2004 Mar 30;321(1):8-22

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中華民國95年06月06日更新