Certificate of Analysis and Data Sheet
Source:
E.Coli
Catalog No.
CYT-458
Introduction:
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptide contains 11 cysteine residues) referred to as the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone 3). Mouse resistin is expressed as a 114 amino acid prepeptide; its hydrofobic Nterminal 20 amino acid signal peptide is cleaved before its secretion. Mouse resistin circulates in blood as a homodimeric protein consisting of two 94 amino acid polypeptides, which are disulfide-linked via Cys26.
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppressed the ability of insulin to stimulate glucose uptake. They have also suggested that resistin was present at elevated levels in blood of obese mice, and was down regulated by fasting and by antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severely suppressed in
obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
Description:
The Recombinant Rat Resistin is manufactured with N-terminal fusion of His tag.
The Recombinant Rat Resistin His-Tagged Fusion Protein is an 11.9 kDa protein containing 94 amino acid residues of the Rat Resistin and 16 additional amino acid residues ?His Tag (bold).
MRGSHHHHHH GMASHMPSMS LCPMDEAISK KINQDFSSLL PAAMKNTVLH CWSVSSRGRL ASCPEGTTVT SCSCGSGCGS WDVREDTMCH CQCGSIDWTA ARCCTLRVGS.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Specificity:
The amino acid sequence of the recombinant Rat Resistin is 100% homologous to the amino acid sequence of the Rat Resistin without signale sequence.
Purification Method:
One -step procedure using affinity Ni-NTA chromatography.
Purity:
>95% (SDS-PAGE analyzed).
Formulation:
Sterile filtered and lyophilized from 0.5 mg/ml in 20mM Tris pH 8.0.
Solubility:
Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
Stability:
Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4蚓 for a limited period of time; it does not show any change after two weeks at 4蚓.
The lyophilized protein remains stable until the expiry date when stored at -20蚓.
Applications:
Western blotting, ELISA
References:
?Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA: The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001).
?Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA: Mechanisms Regulating Adipocyte Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002).
?Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X, Wu GD, Lazar MA: A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506, (2001)
?Way JM, Gצrgn CZ, Tong Q, Uysal KT, Brown KK, Harrington WW, Oliver WR Jr., Willson TM, Kliewer SA, Hotamisligil GS: Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor γ Agonists. J Biol Chem. 276, 25651-25653, (2001).
?Banerjee RR, Lazar MA: Dimerization of resistin and resistin稺ike moleules is etermined by a single cystein. J Biol Chem. 276, 25970-25973, (2001)
?Steppan CM, Lazar MA: Resistin and obesity-associated insulin resistance. TRENDS Endocrinol Metab. 13, 18-23, (2002)
?Pravenec M, Kazdovב L, Landa V, Zםdek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW: Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001)
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Latest Publications:
1. Fat-specific transgenic expression of resistin in the spontaneously hypertensive rat impairs fatty acid re-esterification.
Int J Obes (Lond) 2006 Jan 24;
2. Circulating levels of leptin, adiponectin, resistin, and ghrelin in inflammatory bowel disease.
Inflamm Bowel Dis 2006 Feb;12(2):100-5
3. Up-regulation of PTEN mediates p38 MAPK stress signal-induced inhibition of insulin signaling --- A cross-talk between stress signaling and insulin signaling in resistin-treated human endothelial cells.
J Biol Chem 2006 Jan 17;
4. Changes in fat-derived hormones plasma concentrations: adiponectin, leptin, resistin, and visfatin in rheumatoid arthritis subjects.
Ann Rheum Dis 2006 Jan 13;
5. Resistin as a putative modulator of insulin action in the daily feeding/fasting rhythm.
Pflugers Arch 2006 Jan 11;:1-8
6. Elevated resistin levels in chronic kidney disease are associated with decreas ed glomerular filtration rate and inflammation, but not with insulin resistance.
Kidney Int 2006 Feb;69(3):596-604