Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-398

Description:

Parvalbumin (PV or PVALB or oncomodulin) is a high affinity low mol wt (~12 kDa) cytoplasmic protein belonging to the troponin C superfamily. It is expressed in high levels only in fast-contracting muscles and at lower levels in brain and several endocrine tissues. PV has two isoform: alpha (rat/mouse/human 110-aa, 7p13-p11) beta form (mouse/rat/human 109-aa) shares approx. 48% identity (mouse PVs). The PV-alpha is less acidic (pI=5) than the Beta-isoform and has an additional amino acid in the C-terminal helix. PV-alpha is also expressed in inner ear. PV-beta isoform is expressed in many fetal tissues, placenta and tumors.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris-base, 70mM NaCl, 1mM EDTA and 1mM beta-mercaptoethanol, pH 7.4.

Solubility:

It is recommended to reconstitute the lyophilized Parvalbumin in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Parvalbumin although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution Parvalbumin should be stored at 4⁰C between 2-7 days and for future use below -18⁰C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% as determined by:
Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Applications:

The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For fluid-phase adsorption in immunohistochemistry we suggest the following procedure:
A- Dilute 1 痞 of the mon oclonal antibody 235 (Swant) against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).
B- Add 1ug of the Parvalbumin to 1ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex,DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:Pvalb
Synonyms:Pva

Protein synonyms/aliases:

Parvalbumin alpha.

Protein Family:

Belongs to the parvalbumin family.

Protein Domains:

Domains:
•IPR002048   Calcium-binding EF-hand

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Decrease in parvalbumin-expressing neurons in the hippocampus and increased phencyclidine-induced locomotor activity in the rat methylazoxymethanol (MAM) model of schizophrenia.
Eur J Neurosci 2006 Jan;23(1):279-84
2. Short-term sensory learning does not alter Parvalbumin neurons in the barrel cortex of adult mice: a double-labeling study.
Neuroscience 2006 Jan 10;
3. Functional characterization of parvalbumin from the Arctic cod (Boreogadus saida): Similarity in calcium affinity among parvalbumins from polar teleosts.
Comp Biochem Physiol A Mol Integr Physiol 2006 Jan 10;
4. Parvalbumin correlates with relaxation rate in the swimming muscle of sheepshead and kingfish.
J Exp Biol 2006 Jan 15;209(Pt 2):227-37
5. Pyramidal cells of the rat basolateral amygdala: Synaptology and innervation by parvalbumin-immunoreactive interneurons.
J Comp Neurol 2006 Feb 1;494(4):635-50
6. Parvalbumin deficiency in fast-twitch muscles leads to increased 'slow-twitch type' mitochondria, but does not affect the expression of fiber specific proteins.
FEBS J 2006 Jan;273(1):96-108

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中華民國95年06月06日更新