Source:
E.Coli
Catalog No.
CHM-343
Recombinant Rat MIP-1a is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from 1mg/ml solution containing no additives.
Solubility: It is recommended to reconstitute the lyophilized Rat MIP-1alpha in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized MIP-1alpha although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MIP-1 alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. Amino-Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be
Ala-Pro-Tyr-Gly-Ala. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec旧 Recombinant Rat MIP-1a is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract of rat peritoneal macrophages using a conc.
of 60 ng/ml.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Recombinant MIP-1alpha.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.38 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of MIP-1 alpha as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:Ccl3
Synonyms:Mip1a, Scya3
Small inducible cytokine A3 precursor (CCL3)
(Macrophage inflammatoryprotein 1-alpha)
(MIP-1-alpha).
Belongs to the intercrine beta (chemokine CC) family.
Domains:
•IPR001811 Small chemokine, interleukin-8-like
•Recombinant Human Eotaxin(CCL11)
•Recombinant Murine Eotaxin(CCL11)
•Recombinant Human Monocyte Chemotactic Protein-4 (CCL13)
•Recombinant Human HCC-1 (CCL14)
•Recombinant Human Macrophage Inflammatory protein-4 (CCL18)
•Recombinant Human Macrophage Inflammatory protein-3 beta (CCL19)
•Recombinant Human I-309 (CCL1)
•Recombinant Human Exodus-2 (CCL21)
•Recombinant Human Monocyte Chemotactic Protein-1/MCAF (CCL2)
•Recombinant Murine Monocyte Chemotactic Protein-1 (CCL2)
•Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2)
•Recombinant Human Macrophage Inflammatory protein-1 alpha (CCL3)
•Recombinant Murine Macrophage Inflammatory protein-1 alpha (CCL3)
•Recombinant Human Macrophage Inflammatory protein-1 beta (CCL4)
•Recombinant Murine Macrophage Inflammatory protein-1 beta (CCL4)
•Recombinant Human Rantes (CCL5)
•Recombinant Murine C-10 (CCL6)
•Recombinant Human Monocyte Chemotactic Protein-3 (CCL7)
•Recombinant Murine Monocyte Chemotactic Protein-3 (CCL7)
•Recombinant Human Monocyte Chemotactic Protein-2 (CCL8)
