Source:
E.Coli
Catalog No.
CYT-383
Description : Recombinant Rat IL-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids and having a molecular mass of 15783 Dalton. Recombinant Rat IL-3 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 1mg/ml solution in water containing 5mM sodium citrate pH-4.
Solubility: It is recommended to reconstitute the lyophilized IL-3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Rat IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Interleukin-3 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Asp-Arg-Gly.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec旧 rrIL-3 is fully biologically active when compared to standard.
The ED50 as determined by the dose-dependant stimulation of thymidine uptake by murine MC-9 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 1 x 105 IU/mg.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Recombinant Rat IL-3.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.171 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Rat IL-3 as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:Il3
Synonyms:Il-3
Interleukin-3 precursor (IL-3)
(Multipotential colony-stimulatingfactor)
(Hematopoietic growth factor)
(P-cell-stimulating factor)
(Mast-cell growth factor)
(MCGF).
Belongs to the IL-3 family.
Domains:
•IPR002183 Interleukin-3
•Recombinant Human Interleukin-3
•Recombinant Murine Interleukin-3
