Source:
E.Coli
Catalog No.
CYT-296
GH augments the cytolytic activity of T-cells, antibody synthesis, and granulocyte differentiation induced by GM-CSF . GH also enhances production of TNF-alpha , generation of superoxide anions from peritoneal macrophages, and natural killer activity.
Description: Recombinant Rat GH produced in E.Coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 21940.01 Dalton. Rat-GH is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Rat Growth Hormone(1mg/ml) was lyophilized after extensive dialyses against 5mM phosphate buffer, 5mg mannitol and 1mg glycine. Solubility: It is recommended to reconstitute the lyophilized Rat-GH in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Rat-GH although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Rat-GH should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ile.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Rat Growth Hormone.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of Rat Growth Hormone as a Reference Standard.
References: Flint D. J. & Gardner M.J. (1993) J. Endocrinol., 137, 203-211.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:Gh1
Synonyms:Gh
Somatotropin precursor (Growth hormone).
Belongs to the somatotropin/prolactin family.
Domains:
•IPR001400 Somatotropin hormone
•Recombinant Human Prolactin
•Recombinant Ovine Prolactin
•Recombinant Human Placental-Corr Growth Hormone-22K
•Recombinant Human Pituitary Growth Hormone-20K
•Recombinant Ovine Growth Hormone
•Recombinant bream Growth Hormone
