Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-399

Description:

CABP9K (CALB3 or CABP1; mouse, rat, and human 79 aa; chromosome Xp; ~9 kDa) is a cytosolic Ca-binding protein initially found in rat pancreas. It is also expressed in intestine, placenta, uterus and kidney. Its expression is controlled by vitamin D and sex hormone in a tissue specific manner. In keeping with its role in Ca-transport, its expression is highest in duodenal villus enterocytes. It is further shown that CABP9K is only expressed in differentiated enterocytes. CABP9K gene also contains Cdx2-homeoprotein binding sites, and that Cdx2 may play a crucial role in CABP9K transcription.
CaBP9K is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein was lyophilized from a concentrated solution (1mg/ml) containing 100mM Phosphate buffer, pH 7.3.

Solubility:

It is recommended to reconstitute the lyophilized CABP9K in sterile 18MΩ-cm HO not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized CABP9K although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution CABP9K should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% as determined by:
Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:S100g
Synonyms:Calb3, S100d

Protein synonyms/aliases:

Vitamin D-dependent calcium-binding protein, intestinal (CABP)
    (Calbindin D9K)
    (Cholecalcin).

Protein Family:

Belongs to the S-100 family.

Protein Domains:

Domains:
•IPR002048   Calcium-binding EF-hand
•IPR001751   Calcium-binding protein, S-100/ICaBP type

Related proteins:

•Recombinant Human S100b
•Recombinant Human S100A1
•Recombinant Human S100A4
•Recombinant Human S100A10
•Recombinant Human S100A11

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Complex regulation of Calbindin-D(9k) in the mouse placenta and extra-embryonic membrane during mid- and late pregnancy.
Mol Cell Endocrinol 2004 Feb 12;214(1-2):39-52
2. Effect of age and dietary calcium on intestinal calbindin D-9k expression in the rat.
Arch Biochem Biophys 2003 Dec 1;420(1):194-200
3. Mouse calbindin-D(9k) gene expression in the uterus during late pregnancy and lactation.
Mol Cell Endocrinol 2003 Jul 31;205(1-2):79-88
4. Expression of human Calbindin-D(9k) correlated with age, vitamin D receptor and blood calcium level in the gastrointestinal tissues.
Clin Biochem 2003 Jun;36(4):255-61
5. Novel Calbindin-D(9k) protein as a useful biomarker for environmental estrogenic compounds in the uterus of immature rats.
Reprod Toxicol 2003 May-Jun;17(3):311-9
6. Stimulation of calbindin-D(9k) mRNA expression in the rat uterus by octyl-phenol, nonylphenol and bisphenol.
Mol Cell Endocrinol 2002 Jun 14;191(2):177-86

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中華民國95年06月06日更新