Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-401

Description:

Calretinin/CR/CLB2/CALB2/Calbindin D29K protein (mouse/erat/human 271aa, chromosome 16q22.1, mol wt ~29 kDa) also belongs to the Calbindin family. It is most closely related to CABP28K (~55% identity). It may be alternatively spliced to a C-terminally truncated fragment, Calretinin-22K in some tumor cell lines. Calretinin is highly expressed in the cerebellum, olfactory bulb, and in auditory neurons. Calretinin gene inactivation in mice eliminated long-term potentiation induction in the gentate gyrus and impaired motor coordination.
rrCaBP29K is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris pH-7.4, 50mM sodium chloride and 1mM beta-mercaptoethanol.

Solubility:

It is recommended to reconstitute the lyophilized CABP29K in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized CABP29K although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution CABP29K should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% as determined by:
Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Applications:

This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure:
A- Dilute 1 痞 of the antiserum against calretinin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).
B- Add 1ug of recombinant protein to 1ml of the diluted antibody solution and mix well.
C- Incubate for at least 6 hours in the cold.
D- Apply to tissue-sections and incubate for 3 days.
E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).

As a result, the immunostaining should be strongly reduced or even completely prevented.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:Calb2

Protein synonyms/aliases:

Calretinin (CR).

Protein Family:

Belongs to the calbindin family.

Protein Domains:

Domains:
•IPR002048   Calcium-binding EF-hand

Related proteins:

•Recombinant Rat Calbindin D-28K
•Recombinant Murine Calretinin

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

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中華民國95年06月06日更新