Source:
E.Coli
Catalog No.
CYT-237
GH augments the cytolytic activity of T-cells, antibody synthesis, and granulocyte differentiation induced by GM-CSF . GH also enhances production of TNF-alpha , generation of superoxide anions from peritoneal macrophages, and natural killer activity.
Description : Recombinant Ovine Growth Hormone produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 22015 Dalton. Recombinant Ovine GH is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Ovine-GH was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Solubility: It is recommended to reconstitute the lyophilized Ovine-GH in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Ovine-GH although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Ovine-GH should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. Amino-Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Pro-Ala. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's Ovine Growth Hormone is fully biologically active when compared to standard. The activity as determined by the dose-dependant stimulation of the proliferation FDCP13B9 cells.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of roGH.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.7 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Ovine-GH as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:GH1
Somatotropin precursor (Growth hormone).
Belongs to the somatotropin/prolactin family.
Domains:
•IPR001400 Somatotropin hormone
•Recombinant Human Prolactin
•Recombinant Ovine Prolactin
•Recombinant Human Placental-Corr Growth Hormone-22K
•Recombinant Human Pituitary Growth Hormone-20K
•Recombinant Rat Growth Hormone
•Recombinant bream Growth Hormone
