Source:
E.Coli
Catalog No.
CYT-346
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Solubility: It is recommended to reconstitute the lyophilized Recombinant TPO in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Recombinant TPO although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Recombinant TPO should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. Amino-Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Val-Ala-Pro. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec旧 Recombinant TPO is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of MO7e cells is less then 1 ng/ml, corresponding to a Specific Activity of 1 x 10 6 IU/mg.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of TPO.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a calibrated solution of TPO as a Reference Standard.
Usage: This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.
Gene:
Name:Thpo
Thrombopoietin precursor (Megakaryocyte colony-stimulating factor)
(Myeloproliferative leukemia virus oncogene ligand)
(C-mpl ligand)
(ML)
(Megakaryocyte growth and development factor)
(MGDF).
Belongs to the EPO/TPO family.
Domains:
•IPR001323 Erythropoietin/thrombopoeitin
•Recombinant Human Erythropoietin-alpha
•Recombinant Human Thrombopoietin
