Source:
E.Coli
Catalog No.
CYT-457
Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppressed the ability of insulin to stimulate glucose uptake. They have also suggested that resistin was present at elevated levels in blood of obese mice, and was down regulated by fasting and by antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severely suppressed in obesity and is stimulated by several antidiabetic drugs.
Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
Description: Mouse Resistin is manufactured with signal sequence of phage fd (21 amino acids) and C-terminal fusion of flagTag (10aa). The Recombinant Mouse Resistin Flag-Tagged Fusion Protein is 13.7 kDa protein containing 93 amino acid residues of the Mouse Resistin and 31 additional amino acid residues - signal sequence of phage fd, flagTag.
Amino Acid sequence: MKKLLFAIPL VVPFYSHSTM ASMPLCPIDE AIDKKIKQDF NSLFPNAIKN IGLNCWTVSS RGKLASCPEG TAVLSCSCGS ACGSWDIREE KVCHCQCARI DWTAARCCKL QVASLEDYKD DDDK
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Specificity: The amino acid sequence of the recombinant Mouse Resistin is 100% homologous to the amino acid sequence of the Mouse Resistin.
Purification Method: Two-step procedure using size exclusion chromatography before and after refolding.
Purity: >95% (SDS-PAGE analyzed). Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in 0.05 M Acetate buffer pH4 Reconstitution: Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.
Solubility:
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Stability:
Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4蚓 for a limited period of time; it does not show any change after two weeks at 4蚓.
The lyophilized protein remains stable until the expiry date when stored.
Applications: Western blotting, ELISA
References: ?Steppan CM, Bailley ST, Brown EJ, Banerjee RR, Wright CM, Patel HR, Ahima RS, Lazar MA: The Hormone Links Obesity to Diabetes. Nature. 409, 307-312, (2001)
?Hartman HB, Hu X, Tyler KX, Dalal CK, Lazar MA: Mechanisms Regulating Adipocyte Expression of Resistin. J Biol Chem. 277, 19754-19761, (2002)
?Steppan CM, Brown EJ, Wright CM, Bhat S, Banerjee RR, Dai CY, Enders GH, Silberg DG, Wen X, Wu GD, Lazar MA: A Family of Tissue-specific Resistin-like Molecules. PNAS. 98, 502-506, (2001)
?Way JM, Gצrgn CZ, Tong Q, Uysal KT, Brown KK, Harrington WW, Oliver WR Jr., Willson TM, Kliewer SA, Hotamisligil GS: Adipose Tissue Resistin Expression Is Severly Suppressed in Obesity and Stimulated by Peroxisome Proliferator-activated Receptor γ Agonists. J Biol Chem. 276, 25651-25653, (2001)
?Banerjee RR, Lazar MA: Dimerization of resistin and resistin稺ike moleules is etermined by a single cystein. J Biol Chem. 276, 25970-25973, (2001)
?Steppan CM, Lazar MA: Resistin and obesity-associated insulin resistance. TRENDS Endocrinol Metab. 13, 18-23, (2002)
?Pravenec M, Kazdovב L, Landa V, Zםdek V, Mlejnek P, Jansa P, Wang J, Qi N, Kurtz TW: Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat. J Biol Chem. 278, 45209-45215, (2001)
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:Retn
Synonyms:Fizz3
Resistin precursor (Cysteine-rich secreted protein FIZZ3)
(Adiposetissue-specific secretory factor)
(ADSF)
(Adipose-specific cysteine-rich secreted protein A12-alpha).
Belongs to the resistin/FIZZ family.
Domains:
•IPR009714 Resistin
•Recombinant Mouse Resistin like alpha (RELMalpha)
•Recombinant Human Resistin like beta (RELMbeta/FIZZ2)
•Recombinant Human Resistin (FIZZ3)
•Recombinant Mouse Resistin Like gamma
•Recombinant Rat Resistin
