Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CYT-455

Introduction:

RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats.
Microarray and real-time RT-PCR experiments revealed a repression of RELMgamma mRNA in nasal respiratory epithelium of cigarette smoke-exposed versus untreated rats. The analysis of the physiological tissue-specific expression revealed highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes.
Promyelocytic HL60 cells transfected with a RELM-gamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELM-gamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.

Description:

The Recombinant Mouse RELM-gamma is a His -Tagged Fusion Protein having a molecular weight of 11 kDa containing 86 amino acid residues of the Mouse RELM-gamma and 16 additional amino acid residues ?HisTag (bold).
MRGSHHHHHH GMASHMTLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Specificity:

The amino acid sequence of the recombinant Mouse RELM-gamma is 100% homologous to the amino acid sequence of the Mouse RELM-g.

Purification Method:

Two-step procedure using size exclusion chromatography before and after refolding.

Purity:

>95% (SDS-PAGE analyzed).

Formulation:

Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH 4.

Solubility:

Add 0.2 ml of 0.1M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited.

Stability:

Store lyophilized protein at -20蚓. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4蚓 for a limited period of time; it does not show any change after two weeks at 4蚓.
The lyophilized protein remains stable until the expiry date when stored at -20蚓.

Applications:

Western blotting, ELISA

References:

?Wang ML, Shin ME, Knight PA, Artis D, Silberg DG, Suh E, Wu GD. Regulation of RELM/FIZZ Isoform Expression by Cdx2 in Response to Innate and Adaptive Immune Stimulation in the Intestine. Am J Physiol Gastrointest Liver Physiol. 2004 Dec 2;

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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