Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CYT-351

Background:

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production .In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines . These functions include the regulation of hematopoiesis , angiogenesis , wound healing , inflammation , and immune responses.

Description :

Recombinant Murine Leptin produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16,240 Dalton.
Recombinant Leptin is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant Leptin was lyophilized from a concentrated (1mg/ml) solution with 50mM NH₄HCO₃ (pH 8.0).

Solubility:

The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.

Stability:

Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0 C. Reconstituted Leptin is best stored refrigerated at 4 C.
Please avoid freeze-thaw cycles.

Purity:

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Gln.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's Recombinant mouse Leptin is fully biologically active when compared to standards. Biological activity of Murine Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gram body weight for a period of 14 days. Significant effects on body weight , food consumption, and plasma glucose levels were observed to saline-treated controls.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Leptin.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:Lep
Synonyms:Ob

Protein synonyms/aliases:

Leptin precursor (Obesity factor).

Protein Family:

Belongs to the leptin family.

Protein Domains:

Domains:
•IPR000065   Obesity factor

Related proteins:

•Recombinant Human Leptin
•Recombinant Rat Leptin
•Recombinant Ovine Leptin

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Effect of smoking on neonatal and maternal serum and breast milk leptin levels.
Endocr Res 2005;31(3):177-83
2. Relationship between serum leptin levels and body composition and markers of malnutrition in nondiabetic patients on peritoneal dialysis or hemodialysis.
J Chin Med Assoc 2005 Dec;68(12):566-70
3. Relationship between insulin-like growth factors (IGF-I and IGF-II), IGF-binding proteins (IGFBP-3, IGFBP-2), leptin and anthropometric parameters (height, body mass index) during antileukaemic treatment in children.
Rocz Akad Med Bialymst 2005;50:208-11
4. Body composition, dehydroepiandrosterone sulfate and leptin concentrations in girls approaching menarche.
J Pediatr Endocrinol Metab 2005 Oct;18(10):975-83
5. Leptin levels in boys with pubertal gynecomastia.
J Pediatr Endocrinol Metab 2005 Oct;18(10):929-34
6. Plasma leptin concentrations in postmenopausal women with osteoporosis.
Endocr Res 2005;31(2):133-8

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中華民國95年06月06日更新