Source:
E.Coli
Catalog No.
CHM-313
Murine JE(MCP-1) is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Murine JE(MCP-1) was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives. Solubility: It is recommended to reconstitute the lyophilized Murine JE(MCP-1) in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized Murine JE(MCP-1) although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Murine JE(MCP-1) should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. Amino-Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's Murine JE(MCP-1)is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract Balb/C mouse spleen MNCs at 1.0-20.0 ng/ml.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Murine JE(MCP-1).
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Murine JE(MCP-1) as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:Ccl2
Synonyms:Je, Mcp1, Scya2
Small inducible cytokine A2 precursor (CCL2)
(Monocyte chemotacticprotein 1)
(MCP-1)
(Monocyte chemoattractant protein 1)
(Platelet-derived growth factor-inducible protein JE).
Belongs to the intercrine beta (chemokine CC) family.
Domains:
•IPR001811 Small chemokine, interleukin-8-like
•Recombinant Human Eotaxin(CCL11)
•Recombinant Murine Eotaxin(CCL11)
•Recombinant Human Monocyte Chemotactic Protein-4 (CCL13)
•Recombinant Human HCC-1 (CCL14)
•Recombinant Human Macrophage Inflammatory protein-4 (CCL18)
•Recombinant Human Macrophage Inflammatory protein-3 beta (CCL19)
•Recombinant Human I-309 (CCL1)
•Recombinant Human Exodus-2 (CCL21)
•Recombinant Human Monocyte Chemotactic Protein-1/MCAF (CCL2)
•Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2)
•Recombinant Human Macrophage Inflammatory protein-1 alpha (CCL3)
•Recombinant Murine Macrophage Inflammatory protein-1 alpha (CCL3)
•Recombinant Human Macrophage Inflammatory protein-1 beta (CCL4)
•Recombinant Murine Macrophage Inflammatory protein-1 beta (CCL4)
•Recombinant Human Rantes (CCL5)
•Recombinant Murine C-10 (CCL6)
•Recombinant Human Monocyte Chemotactic Protein-3 (CCL7)
•Recombinant Murine Monocyte Chemotactic Protein-3 (CCL7)
•Recombinant Human Monocyte Chemotactic Protein-2 (CCL8)
