Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-336

Background:

Among CXC chemokines, CXCL10 has been identified to play an important role in several endocrinological autoimmune diseases such as Hashimoto's thyroiditis, Graves'disease and Type 1 diabetes mellitus.The chemokine IP-10 (interferon-inducible protein of 10 kDa, CXCL10) is a chemoattractant for CXCR3+ T cells, binds to the G protein-coupled receptor CXCR3, which is found mainly on activated T cells and NK cells, and plays an important role in Th1-type inflammatory diseases. IP-10 also binds to glycosaminoglycans (GAGs), an interaction thought to be important for its sequestration on endothelial and other cells.

Description :

Recombinant Mouse IP-10 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8701Dalton.
Recombinant Murine IP-10 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

The protein was lyophilized with no additives.

Solubility:

It is recommended to reconstitute the lyophilized Mouse IP-10 in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized IP-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution IP-10 should be stored at 4⁰C between 2-7 days and for future use below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Ile-Pro-Leu-Ala-Arg.

Dimers and aggregates:

Less than 1% as determined by silver stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec旧 Murine IP-10 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract IL-2 activated T cells at 0.1-10 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Mouse IP-10.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.02 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IP-10 as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:Cxcl10
Synonyms:Crg2, Ifi10, Inp10, Scyb10

Protein synonyms/aliases:

Small inducible cytokine B10 precursor (CXCL10)
    (Interferon-gamma-induced protein CRG-2)
    (Gamma-IP10)
    (IP-10)
    (C7).

Protein Family:

Belongs to the intercrine alpha (chemokine CxC)

Protein Domains:

Domains:
•IPR001811   Small chemokine, interleukin-8-like

Related proteins:

•Recombinant Human GRO-alpha (CXCL1)
•Recombinant Mouse GRO/KC (CXCL1)
•Recombinant Human Cytokeratin 18 (CK18)
•Recombinant Human Interleuikin-8 (1-72) (CXCL8)
•Recombinant Human GRO-beta/MIP-2 (CXCL2)
•Recombinant Human GRO-gamma (CXCL3)
•Recombinant Human Epithelial Neutrophil-Activating Protein 78 (CXCL5)
•Recombinant Human Neutrophil Activating Protein-2 (CXCL7)
•Recombinant Human MIG (CXCL9)
•Recombinant Mouse MIG (CXCL9)
•Recombinant Human IP-10 (CXCL10)
•Recombinant Human I-TAC (CXCL11)
•Recombinant Human Stromal Cell-Derived Factor-1 alpha (CXCL12)
•Recombinant Murine Stromal Cell-Derived Factor-1 alpha (CXCL12)

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:
1. Adenovirus-directed ocular innate immunity: the role of conjunctival defensin-like chemokines (IP-10, I-TAC) and phagocytic human defensin-alpha.
Invest Ophthalmol Vis Sci 2005 Oct;46(10):3657-65
2. [Elevated plasma concentration of IP-10 in patients with type 2 diabetes mellitus]
Nippon Jinzo Gakkai Shi 2005;47(5):524-30
3. Cerebrospinal fluid interferon-gamma-inducible protein 10 (IP-10, CXCL10) in HIV-1 infection.
J Neuroimmunol 2005 Nov;168(1-2):154-63
4. TNF-alpha enhances phenotypic and functional maturation of human epidermal Langerhans cells and induces IL-12 p40 and IP-10/CXCL-10 production.
FEBS Lett 2005 Jul 4;579(17):3660-8
5. Synthesis of alpha-chemokines IP-10, I-TAC, and MIG are differentially regulated in human corneal keratocytes.
Invest Ophthalmol Vis Sci 2005 May;46(5):1668-74
6. Human airway epithelial cells produce IP-10 (CXCL10) in vitro and in vivo upon rhinovirus infection.
Am J Physiol Lung Cell Mol Physiol 2005 Jul;289(1):L85-95

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中華民國95年06月06日更新