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Recombinant Murine Eotaxin(CCL11)

Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CHM-308

Description :

Recombinant Murine Eotaxin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8403.2 Dalton.
Murine Eotaxin is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Murine Eotaxin was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized Murine Eotaxin in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Murine Eotaxin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Murine Eotaxin should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Amino-Acid Sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be His-Pro-Gly-Ser-Ile.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's Eotaxin is fully biologically active when compared to standard. The Biological activity is determined by its ability to induce chemotaxis purified eosinophils at a concentration of 100-1000 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Eotaxin.

Protein content:

Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Eotaxin as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


Gene:

Name:Ccl11
Synonyms:Scya11

Protein synonyms/aliases:

Eotaxin precursor (Small inducible cytokine A11)
    (CCL11)
    (Eosinophilchemotactic protein).

Protein Family:

Belongs to the intercrine beta (chemokine CC) family.

Protein Domains:


Domains:
IPR001811   Small chemokine, interleukin-8-like

Related proteins:

Recombinant Human Eotaxin(CCL11)
Recombinant Human Monocyte Chemotactic Protein-4 (CCL13)
Recombinant Human HCC-1 (CCL14)
Recombinant Human Macrophage Inflammatory protein-4 (CCL18)
Recombinant Human Macrophage Inflammatory protein-3 beta (CCL19)
Recombinant Human I-309 (CCL1)
Recombinant Human Exodus-2 (CCL21)
Recombinant Human Monocyte Chemotactic Protein-1/MCAF (CCL2)
Recombinant Murine Monocyte Chemotactic Protein-1 (CCL2)
Recombinant Rat Monocyte Chemotactic Protein-1 (CCL2)
Recombinant Human Macrophage Inflammatory protein-1 alpha (CCL3)
Recombinant Murine Macrophage Inflammatory protein-1 alpha (CCL3)
Recombinant Human Macrophage Inflammatory protein-1 beta (CCL4)
Recombinant Murine Macrophage Inflammatory protein-1 beta (CCL4)
Recombinant Human Rantes (CCL5)
Recombinant Murine C-10 (CCL6)
Recombinant Human Monocyte Chemotactic Protein-3 (CCL7)
Recombinant Murine Monocyte Chemotactic Protein-3 (CCL7)
Recombinant Human Monocyte Chemotactic Protein-2 (CCL8)

Protein links:

Recombinant Murine Eotaxin(CCL11) protein domain

Recombinant Murine Eotaxin(CCL11) protein family


Precursor- Protein structure and amino acid sequence:


Latest Publications:

1. Murine eotaxin-2: a constitutive eosinophil chemokine induced by allergen challenge and IL-4 overexpression.
J Immunol 2000 Nov 15;165(10):5839-46
2. Murine eotaxin: an eosinophil chemoattractant inducible in endothelial cells and in interleukin 4-induced tumor suppression.
Proc Natl Acad Sci U S A 1995 Sep 12;92(19):8960-4

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中華民國95年06月06日更新