Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-290

Background:

Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural mitif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.

Description :

Recombinant Calretinin shows a band at 57kDa on SDS-PAGE commassie blue stained gel. Recombinant Murine Calretinin protein is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered liquid.

Formulation:

100ug/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability:

Store vial at -20C^o to -80C^o. When stored at the recommended temperature, Murine Calretinin protein is stable for 12 months.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates:

Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Murine Calretinin.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:Calb2

Protein synonyms/aliases:

Calretinin (CR).

Protein Family:

Belongs to the calbindin family.

Protein Domains:

Domains:
•IPR002048   Calcium-binding EF-hand

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Calretinin, CD34, and alpha-smooth muscle actin in the identification of peritoneal invasive implants of serous borderline tumors of the ovary.
Mod Pathol 2006 Jan 13;
2. Calretinin immunoreactivity in the brain of the zebrafish, Danio rerio: Distribution and comparison with some neuropeptides and neurotransmitter-synthesizing enzymes. II. Midbrain, hindbrain, and rostral spinal cord.
J Comp Neurol 2006 Feb 10;494(5):792-814
3. Immunohistochemical localization of calbindin-D28k and calretinin in the spinal cord of Xenopus laevis.
J Comp Neurol 2006 Feb 10;494(5):763-83
4. Immunoreactivity for calretinin and calbindin in the vestibular nuclear complex of the monkey.
Exp Brain Res 2005 Dec 21;:1-11
5. Calbindin and calretinin immunoreactivity in the retina of adult and larval sea lamprey.
Brain Res 2006 Jan 12;1068(1):118-30
6. Targeted calretinin expression in granule cells of calretinin-null mice restores normal cerebellar functions.
FASEB J 2005 Dec 13;

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中華民國95年06月06日更新