Certificate of Analysis and Data Sheet

Source:
E.coli

Catalog No.
CYT-432

Description:

Adiponectin/Acrp30 (247amino acids) is adipocyte complement-related protein of 30kDa and exclusively expressed in differentiated adipocytes. Adiponectin(Acrp30) is a member of the complement factor C1q family and consists of signal sequence, Non-homologous sequence, collagen domain and globular domain(gAcrp30).
Adiponectin(Acrp30) expression is reduced in a variety of obese and insulin-resistant states in human, monkeys and mice. Injection of Acrp30(247aa) or gAcrp30(globular domain) lowers serum glucose and free fatty acid level in mice. The globular domain of adiponectin/acrp30(amino acid residues, 111-247, gAcrp30) was overexpressed in E.coli and purified by using conventional chromatography techniques.

Formulation:

1mg/ml solution containing 20mM Tris-HCl pH7.5, 50mM NaCl, 5mM DTT and 10% Glycerol.

Stability:

Liquid rmAcrp30 although stable 14⁰C for 1 week, should be stored desiccated below -18⁰C.
Please prevent freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Sequence:

MAYMYRSAFS VGLETRVTVP NVPIRFTKIF YNQQNHYDGS TGKFYCNIPG LYYFSYHITVYMKDVKVSLF KKDKAVLFTY DQYQEKNVDQ ASGSVLLHLE VGDQVWLQVY GDGDHNGLYADNVNDSTFTG FLLYHDTN

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant Acrp30.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Latest Publications:

1. A longitudinal study of serum adiponectin during normal pregnancy.
BJOG 2006 Jan;113(1):110-3
2. High molecular weight adiponectin correlates with insulin sensitivity in patients with hepatitis C genotype 3, but not genotype 1 infection.
Am J Gastroenterol 2005 Dec;100(12):2717-23
3. Single Nucleotide Polymorphisms in exon 3 of the adiponectin gene in subjects with type 2 diabetes mellitus.
Rocz Akad Med Bialymst 2005;50:148-50
4. Decreased serum adiponectin: an early event in pediatric nonalcoholic fatty liver disease.
J Pediatr 2005 Dec;147(6):835-8
5. Endothelial cell IL-8, a new target for adiponectin: implications in vascular protection.
Circ Res 2005 Dec 9;97(12):1216-9
6. Relationship between plasma adiponectin levels and metabolic risk profiles in Taiwanese children.
Obes Res 2005 Nov;13(11):2014-20