Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-314

Background:

Recombinant Human Ubiquitin (Ub) is a small protein composed of 76 amino acids and having a molecular mass of 8.6kDa. Ub is found only in eukaryotic organisms among which it shows a strong sequence conservation. The protein is present in all cell types, thus giving rise to its name.
Ub is found either in free form or conjugated to proteins through a covalent bond between the glycine at the C-terminal end of Ub and the side chains of lysine on the proteins. The attachment of multiple copies of ubiquitin targets the proteins for degradation by the 26S proteosome. Ubiquitin ligation is an ATP-dependent multi-step process: Ub is activated by the E1 enzyme, the attachment of Ub to the target protein is catalyzed by the E2 enzyme acting in concert with E3 which plays a role in recognizing the substrate protein.

Description :

Recombinant human ubiquitin expressed in Escherichia coli and purified by ion-exchange chromatography.

Physical Appearance:

Sterile filtered liquid.

Formulation:

1mg/ml containing 50mM HEPES (pH7.5) 150mM NaCl, 1mM DTT and 10%glycerol.

Stability:

Store at -20蚓 if intended use is longer than 1 week, store at 4蚓 for a period up to 1 week.
Please avoid freeze-thaw cycles.

Purity:

minimum 95% as estimated by analysis of Coomassie blue stained SDS-PAGE.

Sequence:

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Selected Application References:

?Haas AL, Siepmann TJ (1997) Pathways of ubiquitin conjugation. FASEB J. 11(14):1257-68.
?Hershko A, Ciechanover A (1998) The ubiquitin system. Annu Rev Biochem. 67:425-79.
?Pickart CM (2000) Ubiquitin in chains. Trends Biochem Sci. 25(11):544-8.
?Pickart CM, Eddins MJ (2004) Ubiquitin: structures, functions, mechanisms. Biochim Biophys Acta. 1695(1-3):55-72.
?Roos-Mattjus P, Sistonen L (2004) The ubiquitin-proteasome pathway. Ann Med. 36(4):285-95.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Ubiquitin-binding domains in Y-family polymerases regulate translesion synthesis.
Science 2005 Dec 16;310(5755):1821-4
2. MDM2 promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma protein.
Mol Cell 2005 Dec 9;20(5):699-708
3. Sporadic inclusion-body myositis: a proposed key pathogenetic role of the abnormalities of the ubiquitin-proteasome system, and protein misfolding and aggregation.
Acta Myol 2005 Jul;24(1):17-24
4. Bioinformatic analyses implicate the collaborating meiotic crossover/chiasma proteins Zip2, Zip3, and Spo22/Zip4 in ubiquitin labeling.
Proc Natl Acad Sci U S A 2005 Dec 6;102(49):17594-9
5. Reexamination of the role of ubiquitin-like modifier ISG15 in the phenotype of UBP43-deficient mice.
Mol Cell Biol 2005 Dec;25(24):11030-4
6. Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex.
Mol Cell 2005 Nov 23;20(4):525-38