Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells.

Catalog No.
ENZ-285

Background :

Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called `microsomal antigen` has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100 kDa molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.

Description :

Recombinant Human TPO produced in SF9 is a glycosylated, polypeptide chain coding for the extracellular domain of native TPO, containing 834 amino acids and having a molecular mass of 92,872 dalton. Recombinant TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Formulation:

Recombinant Human TPO is supplied at a of 1.65mg/ml in 16mM sodium phosphate buffer pH-7.6, 400mM sodium chloride, 0.08mM Kl and 20% glycerol.

Immunological functions:

1. Binds IgG-type human auto-antibodies.
2. Auto-antibodies to TPO recognize conformation-dependent epitopes.
3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).

Applications:

Western-Blot with monoclonal anti-TPO antibody and monoclonal anti-hexa-His-tag antibody.

Coating concentration:

0.15-0.375 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Storage Conditions:

Store at 4蚓 if entire vial will be used within 2-4 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:TPO

Protein synonyms/aliases:

Thyroid peroxidase precursor (EC 1.11.1.8)
    (TPO).

Protein Family:

Belongs to the peroxidase family. XPO subfamily.

Protein Domains:

Domains:
•IPR002016   Haem peroxidase, plant/fungal/bacterial
•IPR002007   Animal haem peroxidase
•IPR000436   Sushi/SCR/CCP
•IPR013032   EGF-like region
•IPR013091   EGF calcium-binding
•IPR001881   EGF-like calcium-binding

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Two novel mutations in the thyroid peroxidase gene with goitrous hypothyroidism.
Endocr J 2005 Oct;52(5):643-5
2. [Mutations of the thyroid peroxidase gene]
Nippon Rinsho 2005 Oct;63 Suppl 10:24-9
3. Intrauterine therapy of goitrous hypothyroidism in a boy with a new compound heterozygous mutation (Y453D and C800R) in the thyroid peroxidase gene. A long-term follow-up.
Am J Obstet Gynecol 2005 Sep;193(3 Pt 1):857-8
4. [Thyroid peroxidase antibodies (TPOAb)]
Nippon Rinsho 2005 Aug;63 Suppl 8:284-7
5. Relationship between thyroid peroxidase T cell epitope restriction and antibody recognition of the autoantibody immunodominant region in human leukocyte antigen DR3 transgenic mice.
Endocrinology 2005 Nov;146(11):4961-7
6. Intracellular trafficking of thyroid peroxidase to the cell surface.
J Biol Chem 2005 Jul 29;280(30):27713-8

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中華民國95年06月06日更新