Certificate of Analysis and Data Sheet

Source:
Sf9 Insect Cells

Catalog No.
PRO-375

Description:

Recombinant Human Intrinsic Facor (IF, GIF, Cobalamin/Vitamin B-12 binding transport protein). Recombinant Human IF produced in baculovirus is a glycosylated, polypeptide chain containing having a molecular mass of 44,228 Dalton. Human IF is fused to a hexa-histidine at the C-terminus and purified by proprietary chromatographic techniques for removal of bound Vitamin B-12.

Physical Appearance:

Sterile Filtered colorless solution.

Formulation:

The protein solution contains 20mM HEPES pH-8.

Stability:

Recombinant IF although stable at 10⁰C for 1 week, should be stored desiccated below -18⁰C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

Biological Activity:

Standard ELISA test (checker-board analysis of positive/negative sera panels), immunodot with anti-IF autoantibody positive patient sera. Can be used for solid ELISA and fluid phase diagnostic assays, 0.25-0.65ug/ml. Suitable for biotinylation and iodination.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant Human IF.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:GIF
Synonyms:IFMH

Protein synonyms/aliases:

Gastric intrinsic factor precursor (Intrinsic factor)
    (IF)
    (INF).

Protein Family:

Belongs to the eukaryotic cobalamin transport proteins

Protein Domains:

Domains:
•IPR002157   Eukaryotic cobalamin-binding protein

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. [Immunologic tests: Intrinsic factor antibody]
Nippon Rinsho 2005 Jul;63 Suppl 7:559-61
2. Autoantibodies against intrinsic factor (IF) measured with an ELISA using recombinant human IF as both catching and detecting reagent.
Clin Chem Lab Med 2005;43(4):351-6
3. Hereditary juvenile cobalamin deficiency caused by mutations in the intrinsic factor gene.
Proc Natl Acad Sci U S A 2005 Mar 15;102(11):4130-3
4. Composite organization of the cobalamin binding and cubilin recognition sites of intrinsic factor.
Biochemistry 2005 Mar 8;44(9):3604-14
5. Development and validation of an automated chemiluminometric immunoassay for human intrinsic factor antibodies in serum.
Clin Chem 2005 Jan;51(1):232-5
6. Intrinsic factor studies: II. The effect of gastric juice on the urinary excretion of radioactivity after the oral administration of radioactive vitamin B(12).
J Lab Clin Med 2004 Nov;144(5):268-72; discussion 225-6

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中華民國95年06月06日更新