Source:
E.Coli
Catalog No.
CYT-430
cGH is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 adjusted to pH 8. Solubility: It is recommended to reconstitute the lyophilized cGH in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Stability:
Lyophilized cGH although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution at > 0.1 mg/ml and filter sterilization cGH can be stored at 40C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is recomended.
Please avoid freeze-thaw cycles.
Purity: Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE gel. (c) Analysis by RP-HPLC. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala. Dimers and aggregates: The purified cGH (22K) consists of > 99% monomers as determined by gel-filtration chromatography.
Biological Activity: chGH is fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of cGH (22 K).
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.75 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of others GHs as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:GH
Somatotropin precursor (Growth hormone).
Belongs to the somatotropin/prolactin family.
Domains:
•IPR001400 Somatotropin hormone
•Recombinant Human Prolactin
•Recombinant Ovine Prolactin
•Recombinant Human Placental-Corr Growth Hormone-22K
•Recombinant Human Pituitary Growth Hormone-20K
•Recombinant Rat Growth Hormone
•Recombinant Ovine Growth Hormone
•Recombinant bream Growth Hormone
