Certificate of Analysis and Data Sheet

Source:
Leishmania tarentolae.

Catalog No.
PKA-328

Description :

rbP85a functions as the regulatory subunit of the class IA PI3-kinase isoforms a, b, and d.
It contains two SH2 domains that bind to tyrosine-phosphorylated growth factor receptors or substrate adaptor proteins. It also contains a BH (breakpoint cluster region homology) domain that shows GAP activity towards the small GTPases Rab4, Rab5, Cdc42, Rac1 and to a lesser extend towards Rab6 and Rab11. It was shown that PI3Ka catalytic subunit mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3?phosphorylated phosphoinositides. PI3Ka protein kinase activity has been implicated in IRS-1 serine phosphorylation in insulin-treated adipocytes and in STAT3 and IRS-1 phosphorylation upon activation of the type 1 IFN receptor by IFN-a. Recombinant bovine Phosphoinositide 3-kinase subunit p85a has a molecular weight of 83.5 kDa.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant P85a Supplied as a 1mg/ml solution in 25 mM HEPES, pH 8.0, 25 mM NaCl, 2.5 mM MgCl2 and 50% glycerol.

Storage:

rbP85a although stable at 14蚓 for 1 week, should be stored desiccated below -18蚓. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

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