Certificate of Analysis and Data Sheet

Source:
Corn

Catalog No.
PRO-313

Description :

Recombinant Bovine Trypsin is free from any animal and human sources. % of inhibition is equal to bovine trypsin.

Physical Appearance:

Sterile Filtered lyophilized powder.

Formulation:

The protein was lyophilized without any additives.

Solubility:

It is recommended to reconstitute the lyophilized Trypsin in sterile 18MΩ-cm H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Bovine Trypsin although stable at room temp for 1 week, should be stored desiccated below -18⁰C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Biological Activity:

3,650 Units/mg.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant Trypsin.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Protein synonyms/aliases:

Cationic trypsin precursor (EC 3.4.21.4)
    (Beta-trypsin)

Precursor's functional components:

Alpha-trypsin chain 1
Alpha-trypsin chain 2 (Fragment).

Protein Family:

Belongs to the peptidase S1 family.

Protein Domains:

Domains:
•IPR001254   Peptidase S1 and S6, chymotrypsin/Hap

Related proteins:

•Porcine Enteropeptidase/Enterokinase
•Human Factor IX
•Recombinant Human Tissue Plasminogen Activator
•Recombinant Human Hepatocyte Growth Factor
•Recombinant Human Angiostatin Kringles 1-3

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. [Trypsin-like proteinases and trypsin inhibitors in fruiting bodies of higher fungi]
Prikl Biokhim Mikrobiol 2005 Nov-Dec;41(6):612-5
2. Determination of plasma trypsin-like activity in healthy subjects, patients with mild to moderate alcoholic chronic pancreatitis, and patients with nonjaundice pancreatic cancer.
Dig Dis Sci 2005 Nov;50(11):2165-9
3. Activity of recombinant trypsin isoforms on human proteinase-activated receptors (PAR): mesotrypsin cannot activate epithelial PAR-1, -2, but weakly activates brain PAR-1.
Br J Pharmacol 2005 Dec;146(7):990-9
4. Trypsin is associated with the rotavirus capsid and is activated by solubilization of outer capsid proteins.
J Gen Virol 2005 Nov;86(Pt 11):3143-51
5. Use of a novel washing method combining multiple density gradients and trypsin for removing human immunodeficiency virus-1 and hepatitis C virus from semen.
Fertil Steril 2005 Oct;84(4):1001-10
6. Chemically modified, immobilized trypsin reactor with improved digestion efficiency.
J Proteome Res 2005 Sep-Oct;4(5):1805-13

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中華民國95年06月06日更新