Source:
E.Coli
Catalog No.
CYT-301
Description : Recombinant bovine IGFBP-2 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 284 amino acids and having a molecular mass of 30776 Dalton. Recombinant bovine IGFBP-2 is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: IGFBP-2 was lyophilized with no additives.
Solubility: It is recommended to reconstitute the lyophilized bovine IGFBP-2 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized IGFBP-2 although stable at room temperature for 2 weeks, should be stored desiccated below -18 C. Upon reconstitution bovine IGFBP-2 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: ProSpec's Insulin Like Growth Factor Binding Protein-2 is fully biologically active when compared to standards. The ED50, calculated by IGF-I Binding Assay was found to be less then 10 nM.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of IGFBP-2 .
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of IGFBP-2 as a Reference Standard.
Usage: This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.
Gene:
Name:IGFBP2
Synonyms:BP2
Insulin-like growth factor binding protein 2 precursor (IGFBP-2)
(IBP-2)
(IGF-binding protein 2).
Domains:
•IPR000867 Insulin-like growth factor-binding protein, IGFBP
•IPR000716 Thyroglobulin type-1
