Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-210

Description :

Recombinant Zea Mays Casein Kinase 2 alpha is a non-glycosylated polypeptide having a molecular mass of 39.2 kDa. Recombinant Casein Kinase 2 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Zea Mays CK2-alpha is supplied in 50% glycerol.

Unit definition:

1U = 1痠ol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD.

Storage:

Recombinant CK2-beta should be stored at 4蚓 if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20蚓.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

specific activity > 1U/mg.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Purification and characterization of recombinant protein kinase CK2 from Zea mays expressed in Escherichia coli.
Protein Expr Purif 2003 May;29(1):24-32
2. The crystal structure of the complex of Zea mays alpha subunit with a fragment of human beta subunit provides the clue to the architecture of protein kinase CK2 holoenzyme.
Eur J Biochem 2000 Aug;267(16):5184-90
3. Expression, purification and crystallization of the catalytic subunit of protein kinase CK2 from Zea mays.
Acta Crystallogr D Biol Crystallogr 1998 Jan 1;54 ( Pt 1):143-5
4. Crystal structure of the catalytic subunit of protein kinase CK2 from Zea mays at 2.1 A resolution.
EMBO J 1998 May 1;17(9):2451-62