Certificate of Analysis and Data Sheet

Source:
E. coli

Catalog No.
ENZ-308

Description :

Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74C and exhibits a half-life of 40 minutes at 95C . The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5揍3?direction in the presence of magnesium and also possesses a 5揍3?exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.

Unit Definition:

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25C), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10μg activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul.

10X Reaction Buffer with MgCl2:

500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP.

10X Reaction Buffer without MgCl2:

500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. include: 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution.

Features:

Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions.

Specify Your Own Reaction Conditions:

Choose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with
10X Reaction Buffer containing 15mM MgCl2.

Rely on a Performance-Tested Enzyme:

Our PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account.

Applications:

PCR, 3?A-tailing of blunt ends, compatible with Vectors.

Storage Conditions :

Stable for 5 days at 10⁰C, for longer period of time store at -20⁰C.

Storage Buffer:

Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100.

Quality Control Tests:

PCR, activity, SDS-PAGE/purity, endonuclease/nickase.
Lane1-4: A widely used commercial Taq polymerase in different amount.
Lane5-8: ProSpec Taq at same amount as lane1~4. Target fragment size: 500bp, 25 cycles amplification

References:

Chien, A. et al. (1976) J. Bacteriol. 127, 1550-7.
Kaledin, A.S. et al. (1980) Biokhimiia 45, 644-51.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:polA
Synonyms:pol1

Protein synonyms/aliases:

DNA polymerase I, thermostable (EC 2.7.7.7)
    (Taq polymerase 1).

Protein Family:

Belongs to the DNA polymerase type-A family.

Protein Domains:

Domains:
•IPR002421   5'-3' exonuclease
•IPR001098   DNA-directed DNA polymerase

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Coupled protein domain motion in Taq polymerase revealed by neutron spin-echo spectroscopy.
Proc Natl Acad Sci U S A 2005 Dec 6;102(49):17646-51
2. Taq polymerase reverses inhibition of quantitative real time polymerase chain reaction by humic acid.
Croat Med J 2005 Aug;46(4):556-62
3. The implications of using mutagenic primers in combination with Taq polymerase having proofreading activity.
Biologicals 2004 Jun;32(2):84-7
4. Structure-specific DNA-induced conformational changes in Taq polymerase revealed by small angle neutron scattering.
J Biol Chem 2004 Sep 10;279(37):39146-54
5. Trehalose is a potent PCR enhancer: lowering of DNA melting temperature and thermal stabilization of taq polymerase by the disaccharide trehalose.
Clin Chem 2004 Jul;50(7):1256-9
6. New rulings on the Cetus patent for taq polymerase.
Mol Diagn 2000 Mar;5(1):1-3

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新