Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-334

Background:

Thioredoxins comprise a growing family of proteins that function as general protein-disulfide reductases and are maintained in their reduced active form by the flavoenzyme thioredoxin reductase. Human Trx-1 is mainly a cytosolic protein, although it has been shown to translocate into the nucleus upon certain stimuli and can also be secreted.

Description :

Thioredoxins are small disulphide-containing redox proteins that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol.
It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. This could be especially useful in refolding proteins expressed in E. coli. To this end, thioredoxin has been shown to act as a protein disulfide isomerase.
Its Molecular Weight is 11.9kDa. and the pI is 4.67.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Each mg of protein contains 20mM phosphate buffer pH 7.4.

Solubility:

It is recommended to reconstitute the lyophilized Recombinant TRX in sterile 18MΩ-cm H₂O.

Stability:

Recombinant TRX although stable at 4⁰C for 3 weeks, should be stored desiccated below -18⁰C.
Please avoid freeze-thaw cycles.

Amino acid sequence:

HMSDKIIHL TDDSFDTDVLKADGAIL VDFW AEWCGPCKMIAPILDEI GKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGAL DANLA

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant TRX.

Biological Activity:

TRX activity is assayed by measuring the change in absorbance at 650 nm at 25蚓 using 0.13然 bovine insulin containing 0.33mM DTT (pH 6.5).
The specific activity was found to be 3IU/mg.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:TXN
Synonyms:TRDX, TRX, TRX1

Protein synonyms/aliases:

Thioredoxin (ATL-derived factor)
    (ADF)
    (Surface associated sulphydrylprotein)
    (SASP).

Protein Family:

Belongs to the thioredoxin family.

Protein Domains:

Domains:
•IPR006662   Thioredoxin-related

Related proteins:

•Recombinant Disulfide Oxidoreductase

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Lifespan decrease in a Caenorhabditis elegans mutant lacking TRX-1, a thioredoxin expressed in ASJ sensory neurons.
FEBS Lett 2006 Jan 23;580(2):484-490
2. Coexpression of the subunits of T7 DNA polymerase from an artificial operon allows one-step purification of active gp5/Trx complex.
Protein Expr Purif 2005 Nov 8;
3. Synergistic action of MLL, a TRX protein with template activating factor-I, a histone chaperone.
FEBS Lett 2005 Jan 31;579(3):757-62
4. Effect of melatonin cotreatment against kainic acid on coenzyme Q10, lipid peroxidation and Trx mRNA in rat hippocampus.
Int J Neurosci 2004 Sep;114(9):1085-97
5. Cysteine-dependent immune regulation by TRX and MIF/GIF family proteins.
Immunol Lett 2004 Mar 29;92(1-2):143-7
6. Introduction to Trx-G and Pc-G genes.
Methods Enzymol 2004;377:61-70

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中華民國95年06月06日更新