Certificate of Analysis and Data Sheet

Source:
Saccharomyces cerevisiae

Catalog No.
HSP-104

Description :

Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 can protect yeast cells against high temperature and high concentration of ethanol but mutation studies have shown this protein is not required for normal growth. Hsp104 was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
Recombinant HSP-104 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 908 amino acids and having a molecular mass of 102 kDa.

Physical Appearance:

Sterile filtered clorless solution.

Formulation:

The protein (1mg/ml) contains 20mM Tris-HCl, pH 7.4,100mM NaCl. 2mM EDTA and 5% Glycerol.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:HSP104
OrderedLocusNames:YLL026W
ORFNames:L0948

Protein synonyms/aliases:

Heat shock protein 104.

Protein Family:

Belongs to the clpA/clpB family.

Protein Domains:

Domains:
•IPR004176   Clp, N terminal
•IPR003959   AAA ATPase, central region

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Novel vaccines for the treatment of chronic HBV infection based on mycobacterial heat shock protein 70.
Vaccine 2006 Jan 18;
2. Activation of the AMP-Activated Kinase by Antidiabetes Drug Metformin Stimulates Nitric Oxide Synthesis In Vivo by Promoting the Association of Heat Shock Protein 90 and Endothelial Nitric Oxide Synthase.
Diabetes 2006 Feb;55(2):496-505
3. Association between heat shock protein 70s and Toll-like receptor polymorphisms with long-term renal allograft survival.
Transpl Int 2006 Mar;19(3):190-6
4. [Heat shock protein 70 expression in relation to apoptosis in primary bladder transitional cell carcinoma.]
Chin Med J (Engl) 2005 Dec 20;118(24):2093-6
5. [Increased expression of 70 kD heat shock protein in cultured primary human keratinocytes induced by human papillomavirus 16 E6/E7 gene.]
Chin Med J (Engl) 2005 Dec 20;118(24):2058-62
6. Use of a Commercial Assay for Detecting the 60 kDa Chlamydial Heat Shock Protein in a Range of Patient Groups.
Sex Transm Dis 2006 Feb;33(2):77-79

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中華民國95年06月06日更新