Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-356

Background:

Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in E.coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of Staphylococcus endotoxins and hemolysin).

Description :

Recombinant Staphylococal Protein A produced in E.Coli is a non-glycosylated, Polypeptide chain containing 178 amino acids and having a molecular mass of 23,307 Dalton.
Recombinant Staphylococal Protein A is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant Protein A was lyophilized from a concentrated (1mg/ml) solution containing no additives.

Stability:

Recombinant Protein A although stable at 4 C for 21 days, should be stored desiccated below -20 C for long term periods .
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Recombinant Protein A.

Activity:

Greater than 95% binding activity to human IgG.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:spa

Protein synonyms/aliases:

Immunoglobulin G binding protein A precursor (IgG binding protein A)
    (Staphylococcal protein A).

Protein Family:

Protein Domains:

Domains:
•IPR005877   YSIRK Gram-positive signal peptide
•IPR003132   Ig-binding B
•IPR005038   Octapeptide repeat
•IPR005038   Octapeptide repeat
•IPR004829   Cell surface antigen
•IPR005038   Octapeptide repeat
•IPR002482   Peptidoglycan-binding LysM
•IPR001899   Surface protein from Gram-positive cocci, anchor region

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Staphylococcal protein a deletes B-1a and marginal zone B lymphocytes expressing human immunoglobulins: an immune evasion mechanism.
J Immunol 2005 Dec 1;175(11):7719-27
2. On the mechanism of staphylococcal protein A immunomodulation.
Transfusion 2005 Feb;45(2):274-80
3. Acute glomerulonephritis occurring during immunoadsorption with staphylococcal protein A column (Prosorba).
Nephrol Dial Transplant 2004 Dec;19(12):3155-9
4. The role of circulating immune complexes and biocompatibility of staphylococcal protein A immunoadsorption in mitomycin C-induced hemolytic uremic syndrome.
Am J Kidney Dis 2004 Oct;44(4):e50-8
5. Staphylococcal protein A inflames the lungs.
Nat Med 2004 Aug;10(8):780-1
6. Use of staphylococcal protein A in the analysis of teleost immunoglobulin structural diversity.
Dev Comp Immunol 2004 Jun;28(7-8):803-14

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中華民國95年06月06日更新