Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-396

Background:

SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release . The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane.

Description :

SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.
Recombinant C.elegans SNAP-25 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23kDa.

Physical Appearance:

Sterile filtered clorless solution.

Formulation:

The protein (1mg/ml) contains 20mM Tris-HCl pH7.5, 50mM NaCl, 5mMDTT, 1mM EDTA and 10%Glycerol.

Stability:

Store at 4蚓 if entire vial will be used within 2-4 weeks.
Store, frozen at -20蚓 for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.

Sequence:

MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERCEGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQPRITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Stable silencing of SNAP-25 in PC12 cells by RNA interference.
BMC Neurosci 2006 Jan 30;7(1):9
2. Group IIA phospholipase A(2) is coexpressed with SNAP-25 in mature taste receptor cells of rat circumvallate papillae.
J Comp Neurol 2006 Feb 20;494(6):876-86
3. Selective effects of typical antipsychotic drugs on SNAP-25 and synaptophysin in the hippocampal trisynaptic pathway.
Int J Neuropsychopharmacol 2005 Sep 5;:1-7
4. A fission yeast SNAP-25 homologue, SpSec9, is essential for cytokinesis and sporulation.
Cell Struct Funct 2005;30(2):15-24
5. Dynamic association of the Ca2+ channel alpha1A subunit and SNAP-25 in round or neurite-emitting chromaffin cells.
Eur J Neurosci 2005 Nov;22(9):2187-98
6. The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells.
J Biol Chem 2005 Nov 25;280(47):39253-9