Certificate of Analysis and Data Sheet

Source:
Insect Cells

Catalog No.
SIV-114

Description :

Recombinant SIVp55, Strains: SIV mac 23g and SIV smH4 is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. SIV-1 p66 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Recombinant SIV-1 p55 was lyophilized from (0.1mg/ml) sterile solution containing 10mM Tris, pH 8, 140mM NaCl and 400mM L-Arginine.

Solubility:

It is recommended to reconstitute the lyophilized SIV-p55 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized SIV-p55 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution SIV-p55 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of SIV-p55.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.