Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ALR-002

Background:

Description:

E.coli derived recombinant. The protein contains Soy Bean P34 Protein epitopes (214-261, 351-371 a.a).

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein concentration:

1 mg/ml containing 50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability:

Five years frozen. One month in solution at room temperature.

Application:

Use as an antigen in ELISA and Western Blots.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Protein synonyms/aliases:

P34 probable thiol protease precursor (EC 3.4.22.-).

Protein Family:

Belongs to the peptidase C1 family.

Protein Domains:

Domains:
•IPR000668   Peptidase C1A, papain C-terminal
•IPR013128   Peptidase C1A, papain

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Allergenic proteins in soybean: processing and reduction of P34 allergenicity.
Nutr Rev 2005 Feb;63(2):47-58
2. The P34 syringolide elicitor receptor interacts with a soybean photorespiration enzyme, NADH-dependent hydroxypyruvate reductase.
Mol Plant Microbe Interact 2002 Dec;15(12):1213-8
3. A soybean vacuolar protein (P34) related to thiol proteases is synthesized as a glycoprotein precursor during seed maturation.
J Biol Chem 1992 Jun 15;267(17):12068-76

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中華民國95年06月06日更新