Source:
E.Coli
Catalog No.
ENZ-288
Staphylokinase is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Recombinant SAK was lyophilized from a sterile solution containing 20mM phosphate buffer pH-7.
Solubility: It is recommended to reconstitute the lyophilized SAK in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized SAK although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution SAK should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained. Biological Activity: The biological activity measured by the ability of fibrin lysis in agarose plate was found to be 4600IU/mg.
Amino-Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ser-Ser-Phe-Asp. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Staphylokinase.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a calibrated solution of Staphylokinase as a Reference Standard.
Usage: Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Gene:
Name:sak
Staphylokinase precursor (SakSTAR)
(Neutral proteinase)
(ProteaseIII).
Belongs to the staphylokinase family.
Domains:
•IPR004093 Staphylokinase/Streptokinase
