Source:
E.Coli
Catalog No.
PRO-323
Description : Recombinant Rabbit TNC produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 18,020 Dalton. Recombinant Cardiac Troponin-C is purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered lyophilized powder.
Formulation: Lyophilized from (1mg/ml) solution containing 150mM NaCl, 10mM sodium phosphate, 0.5mM EDTA and 0.02% NaN3, (pH 7.0).
Solubility: It is recommended to reconstitute the lyophilized TNC in sterile 18M?cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Stability:
Lyophilized TNC although stable at room temperature for 3 weeks, should be stored desiccated below -18蚓. Upon reconstitution TNC should be stored at 4蚓 between 2-7 days and for future use below -18蚓. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Purity: Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin: Less than 0.1 ng/ug (IEU/ug) of Rabbit TNC.
Protein content: Protein quantitation was carried out by:
1-10 mg/ml Bradford assay vs. BSA
Usage: This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.
Gene:
Name:TNNC1
Troponin C, slow skeletal and cardiac muscles (TN-C).
Belongs to the troponin C family.
Domains:
•IPR002048 Calcium-binding EF-hand
•Recombinant Human Cardiac Troponin-C
