Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-203

Description :

Inactive holoenzyme consisting of one dimeric regulatory subunit type I alpha and two monomeric catalytic subunits (cAMP-free). Recombinant Protein Kinase A is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant PKA holoenzyme type-I alpha is supplied in 50% glycerol.

Storage:

Recombinant PKA should be stored at 4蚓 if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20蚓.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

Prospec's recombinant Holo enzyme can be activated by adding the second messenger cAMP (Activation constant about 100nM) releasing two monomeric catalytic subunits.

Applications:

The product is suitable for analysing PKA type I agonists (cAMP analogs) or antagonists.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Mapping intersubunit interactions of the regulatory subunit (RIalpha) in the type I holoenzyme of protein kinase A by amide hydrogen/deuterium exchange mass spectrometry (DXMS).
J Mol Biol 2004 Jul 23;340(5):1185-96
2. Human myometrial quiescence and activation during gestation and parturition involve dramatic changes in expression and activity of particulate type II (RII alpha) protein kinase A holoenzyme.
J Clin Endocrinol Metab 2003 May;88(5):2194-205