Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
ALR-001

Background:

Description:

E.coli derived recombinant.
The protein contains phospholipase A2 bee venom protein epitope, amino acids: (26-162).

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein concentration:

1 mg/ml containing 10mM Tris-HCl, pH 6.5, 100mM Sodium Phosphate and 8M urea.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability:

Five years frozen. One month in solution at room temperature.

Application:

Use as an antigen in ELISA and Western Blots.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Protein synonyms/aliases:

Phospholipase A2 precursor (EC 3.1.1.4)
    (Phosphatidylcholine 2-acylhydrolase)
    (Allergen Api m 1)
    (Api m I).

Protein Family:

Belongs to the phospholipase A2 family.

Protein Domains:

Domains:
•IPR013090   Phospholipase A2, active site
•IPR008774   A2 Phospholipase
•IPR001211   Phospholipase A2

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Attenuation of the pulmonary inflammatory response following butylated hydroxytoluene (BHT) treatment of cytosolic phospholipase A2 (cPLA2) null mice.
Am J Physiol Lung Cell Mol Physiol 2006 Jan 27;
2. Role of cytochrome P450 in phospholipase A2- and arachidonic acid-mediated cytotoxicity.
Free Radic Biol Med 2006 Feb 1;40(3):364-75
3. Gestation Age-Related Increase in 50 kDa Rat Uterine Calcium-Independent Phospholipase A2 Expression Influences Uterine Sensitivity to Polychlorinated Biphenyl Stimulation.
Biol Reprod 2006 Jan 25;
4. Phospholipase A2 Functions in Pseudomonas aeruginosa- Induced Apoptosis.
Infect Immun 2006 Feb;74(2):850-60
5. Activation of Synoviocytes by the Secreted Phospholipase A2 Motif in the VP1-Unique Region of Parvovirus B19 Minor Capsid Protein.
J Infect Dis 2006 Feb 15;193(4):582-90
6. Group V secretory phospholipase A2 translocates to the phagosome after zymosan stimulation of mouse peritoneal macrophages and regulates phagocytosis.
J Biol Chem 2006 Jan 1;

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中華民國95年06月06日更新