Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
CYT-240

Background:

Prolactin is a lactogenic hormone secreted by the adenohypophysis .Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation.Prolactin has been shown also to have cytokine-like activities and to have important immunoregulatory activities. It contributes to the development of lymphoid tissues and the maintenance of physiological immune function and also modulates a variety of T-cell immune responses. Prolactin has been reported to activate cellular proliferation in nonreproductive tissue, such as liver, spleen, and thymus. It induces significant proliferation in aortic smooth muscle cells and also enhances proliferation of these cells induced by PDGF . Prolactin also appears to be directly mitogenic for pancreatic beta cells. Prolactin is also mitogenic for cultured astrocytes.

Description :

Recombinant Ovine Prolactin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23007 Dalton.
Ovine Prolactin is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Ovine Prolactin was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

Solubility:

It is recommended to reconstitute the lyophilized Ovine Prolactin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized Ovine Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Ovine Prolactin should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity:

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

ProSpec's Ovine Prolactin is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be < 0.065 ng/ml.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Ovine Prolactin.

Protein content:

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Ovine Prolactin as a Reference Standard.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:PRL

Protein synonyms/aliases:

Prolactin precursor (PRL).

Protein Family:

Belongs to the somatotropin/prolactin family.

Protein Domains:

Domains:
•IPR001400   Somatotropin hormone

Related proteins:

•Recombinant Human Prolactin
•Recombinant Human Placental-Corr Growth Hormone-22K
•Recombinant Human Pituitary Growth Hormone-20K
•Recombinant Rat Growth Hormone
•Recombinant Ovine Growth Hormone
•Recombinant bream Growth Hormone

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Large-scale preparation of recombinant ovine prolactin and determination of its in vitro and in vivo activity.
Protein Expr Purif 2001 Aug;22(3):489-96
2. Ovine placental lactogen and ovine prolactin: partial proteolysis and conformational stability.
Int J Biochem Cell Biol 2000 Jun;32(6):597-608
3. In vitro expression of long and short ovine prolactin receptors: activation of Jak2/STAT5 pathway is not sufficient to account for prolactin signal transduction to the ovine beta-lactoglobulin gene promoter.
J Mol Endocrinol 1999 Oct;23(2):125-36
4. Rapid communication: a restriction fragment length polymorphism in the ovine Prolactin gene.
J Anim Sci 1997 Jun;75(6):1686
5. Ovine prolactin increases hepatic UDP-glucuronosyltransferase activity in ovariectomized rats.
J Pharmacol Exp Ther 1996 Aug;278(2):921-5
6. Modulation of prolactin receptors in the rat hypothalamus in response to changes in serum concentration of endogenous prolactin or to ovine prolactin administration.
Brain Res 1994 Nov 14;663(2):244-50

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中華民國95年06月06日更新