Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
MEV-001

Background:

Description:

E.coli derived recombinant.
The protein contains the large polymerase immunodominant regions,amino acids: (58-149)

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein concentration:

1 mg/ml containing 50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione, 0.1% sarcosil and 50% glycero1.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability:

Five years frozen. One month in solution at room temperature.

Application:

Use as an antigen in ELISA and Western Blots.
Immunoreactive with sera of Measles Virus infected individuals.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:L

Protein synonyms/aliases:

Large structural protein (L protein)
    (Transcriptase)
    (Replicase)[Includes: RNA-directed RNA polymerase (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase (EC 2.1.1.56)
mRNA guanylyltransferase(EC 2.7.7.-) .

Protein Family:

Belongs to the paramyxoviruses L protein family.

Protein Domains:

Domains:
•IPR001016   Viral RNA-dependent RNA polymerase, L protein

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. The phosphoprotein (P) and L binding sites reside in the N-terminus of the L subunit of the measles virus RNA polymerase.
Virology 2004 Oct 1;327(2 ):297-306
2. [Diagnosis of adult measles pneumonia from bronchoalveolar lavage fluid by reverse-transcriptase polymerase chain reaction]
Kansenshogaku Zasshi 2003 Jul;77(7):521-5
3. Modulating the function of the measles virus RNA-dependent RNA polymerase by insertion of green fluorescent protein into the open reading frame.
J Virol 2002 Jul;76(14):7322-8
4. Activity of polymerase proteins of vaccine and wild-type measles virus strains in a minigenome replication assay.
J Virol 2002 Jul;76(14):7073-81
5. Restriction of measles virus RNA synthesis by a mouse host cell line: trans-complementation by polymerase components or a human cellular factor(s).
J Virol 2002 Jun;76(12):6121-30
6. Prolonged measles virus shedding in human immunodeficiency virus-infected children, detected by reverse transcriptase-polymerase chain reaction.
J Infect Dis 2001 Feb 15;183(4):532-8

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新