Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
MEV-007

Background:

Description:

E.coli derived recombinant.
The protein contains the large hemagglutinin immunodominant regions,amino acids: (106-114/519-550).

Purification method:

Purified by proprietary chromatographic technique.

Purity:

Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.

Protein concentration:

1 mg/ml containing 50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycero1.

Storage:

Protein is shipped at ambient temperature. Upon arrival, Store at -20⁰C.

Stability:

Five years frozen. One month in solution at room temperature.

Application:

Use as an antigen in ELISA and Western Blots.
Immunoreactive with sera of Measles Virus infected individuals.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:H

Protein synonyms/aliases:

Hemagglutinin glycoprotein.

Protein Family:

Belongs to the paramyxoviruses hemagglutinin-

Protein Domains:

Domains:
•IPR000665   Hemagglutinin-neuraminidase

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Relative contributions of measles virus hemagglutinin- and fusion protein-specific serum antibodies to virus neutralization.
J Virol 2005 Sep;79(17):11547-51
2. Interactions among measles virus hemagglutinin, fusion protein and cell receptor signaling lymphocyte activation molecule (SLAM) indicating a new fusion-trimer model.
J Biochem Mol Biol 2005 Jul 31;38(4):373-80
3. Characterization of immune responses induced by intramuscular vaccination with DNA vaccines encoding measles virus hemagglutinin and/or fusion proteins.
J Virol 2005 Aug;79(15):9854-61
4. Mechanism of CD150 (SLAM) down regulation from the host cell surface by measles virus hemagglutinin protein.
J Virol 2004 Sep;78(18):9666-74
5. Measles virus (MV) hemagglutinin: evidence that attachment sites for MV receptors SLAM and CD46 overlap on the globular head.
J Virol 2004 Sep;78(17):9051-63
6. Selectively receptor-blind measles viruses: Identification of residues necessary for SLAM- or CD46-induced fusion and their localization on a new hemagglutinin structural model.
J Virol 2004 Jan;78(1):302-13

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中華民國95年06月06日更新