Certificate of Analysis and Data Sheet

Source:
Pichia pastoris

Catalog No.
PRO-361

Description :

Recombinant LALF produced in Pichia pastoris ,is a single non-glycosylated single polypeptide chain containing 101 amino acids and having a molecular mass of ~12 kDa. Recombinant LALF is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:

Lyophilized from a concentrated (1mg/ml) solution containing no additives.

Solubility:

It is recommended to reconstitute the lyophilized rLALF in sterile 20mM acetic acid not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Stability:

Lyophilized rLALF although stable at room temperature for 3 weeks, should be stored desiccated below -18⁰C. Upon reconstitution rLALF should be stored at 4⁰C between 2-7 days and for future use below -18⁰C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of LALF.

Biological Activity:

Antibacterial activity against E. coli less than 12 ug/ml at 10⁶ PFU/ml. Binds to and inactivates endotoxin (LPS).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

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Protein synonyms/aliases:

Anti-lipopolysaccharide factor (anti-LPS)
    (LALF).

Protein Family:

Protein Domains:

Domains:

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Cyclic antimicrobial peptides based on Limulus anti-lipopolysaccharide factor for neutralization of lipopolysaccharide.
Biochem Pharmacol 2004 Oct 1;68(7):1297-307
2. Tachyplesin and anti-lipopolysaccharide factor.
Methods Mol Biol 1997;78:63-71
3. High affinity endotoxin-binding and neutralizing peptides based on the crystal structure of recombinant Limulus anti-lipopolysaccharide factor.
J Biol Chem 1996 Nov 8;271(45):28120-7
4. Primary structures and functions of anti-lipopolysaccharide factor and tachyplesin peptide found in horseshoe crab hemocytes.
Adv Exp Med Biol 1990;256:273-85
5. Primary structure of anti-lipopolysaccharide factor from American horseshoe crab, Limulus polyphemus.
J Biochem (Tokyo) 1987 Jun;101(6):1321-30
6. Primary structure of limulus anticoagulant anti-lipopolysaccharide factor.
J Biol Chem 1986 Jun 5;261(16):7357-65

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中華民國95年06月06日更新