Certificate of Analysis and Data Sheet

Source:
Sf9 insect cells.

Catalog No.
ENZ-303

Background :

Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig.
Tissue-type transglutaminase antigens have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme's secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aggregates of tTG antigen and host cell proteins.

Description :

Recombinant Human Tissue Transglutaminase produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 78,018 dalton.
rHutTG is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
By point mutation of the active center the catalytic transglutaminase activity has been eliminated, resulting in increased stability during storage and coating.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human t-TG is supplied at a of 0.5mg/ml in 16mM HEPES buffer pH-7.6, 240mM sodium chloride, and 20% glycerol.

Stability:

Store at 4蚓 if entire vial will be used within 1-2 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.

Immunological functions:

1. Binds IgA & IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).

Applications:

Western-Blot with monoclonal anti-hexa-His-tag antibody.

Coating concentration:

0.6-1.4 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.

Purity:

Greater than 95% (SDS-PAGE).

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Gene:

Name:TGM2

Protein synonyms/aliases:

Protein-glutamine gamma-glutamyltransferase 2 (EC 2.3.2.13)
    (Tissuetransglutaminase)
    (TGase C)
    (TGC)
    (TG(C))
    (Tranglutaminase-2)
    (TGase-H).

Protein Family:

Belongs to the transglutaminase family.

Protein Domains:

Domains:
•IPR001102   Protein-glutamine gamma-glutamyltransferase
•IPR001102   Protein-glutamine gamma-glutamyltransferase
•IPR002931   Transglutaminase-like
•IPR001102   Protein-glutamine gamma-glutamyltransferase

Related proteins:

•Recombinant Human TransGlutaminase II

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. [Tissue transglutaminase and renal fibrosis]
Sheng Li Ke Xue Jin Zhan 2005 Oct;36(4):314-8
2. Phosphorylation of histones by tissue transglutaminase.
J Biol Chem 2006 Jan 4;
3. Antibodies to recombinant human tissue-transglutaminase in coeliac disease: Diagnostic effectiveness and decline pattern after gluten-free diet.
Dig Liver Dis 2006 Feb;38(2):98-102
4. Site-specific transamidation and deamidation of the small heat-shock protein Hsp20 by tissue transglutaminase.
Proteins 2005 Dec 29;
5. Tissue transglutaminase serves as an inhibitor of apoptosis by cross-linking caspase 3 in thapsigargin-treated cells.
Mol Cell Biol 2006 Jan;26(2):569-79
6. Detection of secretory IgA antibodies against gliadin and human tissue transglutaminase in stool to screen for coeliac disease in children: validation study.
BMJ 2006 Jan 28;332(7535):213-4

採購找生工，預算最輕鬆。

回頁首↑ | 回首頁←

---

中華民國95年06月06日更新