Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-301

Background:

p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein p53 senses DNA damage and possibly facilitating repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Description :

Recombinant Mutant p53 shows a band at 81kDa on SDS-PAGE commassie blue stained gel. Recombinant Mutant p53 purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered liquid.

Formulation:

100ug/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability:

Store vial at -20C^o to -80C^o. When stored at the recommended temperature, Mutant p53 is stable for 12 months.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates:

Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human Mutant p53.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals

Gene:

Name:MBP1

Protein synonyms/aliases:

Mutant p53 binding protein 1 (MBP1).

Protein Family:

Protein Domains:

Domains:
•IPR001881   EGF-like calcium-binding
•IPR013091   EGF calcium-binding
•IPR013032   EGF-like region
•IPR013091   EGF calcium-binding
•IPR001881   EGF-like calcium-binding
•IPR013032   EGF-like region
•IPR001881   EGF-like calcium-binding
•IPR013091   EGF calcium-binding
•IPR001881   EGF-like calcium-binding

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Meta-analysis of the p53 mutation database for mutant p53 biological activity reveals a methodologic bias in mutation detection.
Clin Cancer Res 2006 Jan 1;12(1):62-9
2. The differential effects of mutant p53 alleles on advanced murine lung cancer.
Cancer Res 2005 Nov 15;65(22):10280-8
3. Localization of a mutant p53 response element on the Tissue Inhibitor of Metalloproteinase-3 promoter: Mutant p53 activities are distinct from wild-type.
Cancer Lett 2005 Oct 15;
4. Mutant p53 melanoma cell lines respond differently to CP-31398-induced apoptosis.
Br J Dermatol 2005 Nov;153(5):900-10
5. Single chain antibody against the common epitope of mutant p53 restores wild-type activity to mutant p53 protein.
FEBS Lett 2005 Oct 24;579(25):5609-15
6. Mutant p53 gain of function: reduction of tumor malignancy of human cancer cell lines through abrogation of mutant p53 expression.
Oncogene 2006 Jan 12;25(2):304-9

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中華民國95年06月06日更新