Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-302

Background:

p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein p53 senses DNA damage and possibly facilitating repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

Description :

Recombinant Human p53 protein shows a band at 81kDa on SDS-PAGE commassie blue stained gel. Recombinant human p53 protein purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered liquid.

Formulation:

100ug/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability:

Store vial at -20C^o to -80C^o. When stored at the recommended temperature, p53 protein is stable for 12 months.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates:

Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human p53.

Usage:

This material is offered by ProSpec-TechnoGene for research and laboratory use.

Gene:

Name:TP53
Synonyms:P53

Protein synonyms/aliases:

Cellular tumor antigen p53 (Tumor suppressor p53)
    (Phosphoprotein p53)
    (Antigen NY-CO-13).

Protein Family:

Belongs to the p53 family.

Protein Domains:

Domains:
•IPR011615   p53, DNA-binding
•IPR010991   p53, tetramerisation

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

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中華民國95年06月06日更新