Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PKA-217

Description :

Recombinant p38/SAPK2 is a non-glycosylated polypeptide produced by phosphorylation of the purified p38 alpha with MKK6 having a molecular mass of 42.7kDa. Recombinant p38/SAPK2 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile Filtered clear soultion.

Formulation:

Recombinant Human p38/SAPK2 is supplied in 50% glycerol.

Unit definition:

1 U = 1 pmol/min transferred to myelin basic protein at 30 ^o C

Storage:

Recombinant p38/SAPK2 should be stored at 4蚓 if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20蚓.
Please avoid freeze-thaw cycles.

Purity:

Greater than 95.0% as determined by:
(a) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:

Specific activity > 1x10⁵ U/mg.

Usage:

This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.

Latest Publications:

1. Promyelocytic leukemia is a direct inhibitor of SAPK2/p38 mitogen-activated protein kinase.
J Biol Chem 2004 Sep 24;279(39):40994-1003
2. p38/SAPK2 controls gap junction closure in astrocytes.
Glia 2004 May;46(3):323-33
3. Synthetic anisomycin analogues activating the JNK/SAPK1 and p38/SAPK2 pathways.
Org Biomol Chem 2004 Jan 7;2(1):142-9
4. Phosphorylation of tyrosine 1214 on VEGFR2 is required for VEGF-induced activation of Cdc42 upstream of SAPK2/p38.
Oncogene 2004 Jan 15;23(2):434-45
5. Integrin alphavbeta3, requirement for VEGFR2-mediated activation of SAPK2/p38 and for Hsp90-dependent phosphorylation of focal adhesion kinase in endothelial cells activated by VEGF.
Cell Stress Chaperones 2003 Spring;8(1):37-52
6. Transendothelial migration of colon carcinoma cells requires expression of E-selectin by endothelial cells and activation of stress-activated protein kinase-2 (SAPK2/p38) in the tumor cells.
J Biol Chem 2001 Sep 7;276(36):33762-72