Certificate of Analysis and Data Sheet

Source:
E.Coli

Catalog No.
PRO-300

Background:

p21/WAF1/Cip1/Sdi1/Pic1 is a tumor suppressor protein. Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21/WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. Meanwhile, the p21WAF1 protein also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process. Evidently, inactivation of p53 disrupts the p53-dependent expression of p21WAF1. p21WAF1is a tumor suppressor involved in the pathogenesis of a variety of malignancies.

Description :

Recombinant P21/WAF1 shows a band at 47kDa on SDS-PAGE commassie blue stained gel. Recombinant P21/WAF1 is purified by proprietary chromatographic techniques.

Physical Appearance:

Sterile filtered liquid.

Formulation:

100ug/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.

Stability:

Store vial at -20C^o to -80C^o. When stored at the recommended temperature, recombinant P21/WAF1 is stable for 12 months.
Please avoid freeze-thaw cycles.

Purity:

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by reducing and non-reducing SDS-PAGE coomassie blue gel.

Dimers and aggregates:

Less than 1% as determined by coomassie blue SDS-PAGE gel analysis.

Endotoxin:

Less than 0.1 ng/ug (IEU/ug) of Human P21/WAF1.

Usage:

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Gene:

Name:CDKN1A
Synonyms:CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1

Protein synonyms/aliases:

Cyclin-dependent kinase inhibitor 1 (p21)
    (CDK-interacting protein 1)
    (Melanoma differentiation-associated protein 6)
    (MDA-6).

Protein Family:

Belongs to the CDI family.

Protein Domains:

Domains:
•IPR003175   Cyclin-dependent kinase inhibitor

Protein links:

Precursor- Protein structure and amino acid sequence:

Latest Publications:

1. Regulation of the human p21(waf1/cip1) gene promoter via multiple binding sites for p53 and the vitamin D3 receptor.
Nucleic Acids Res 2006 Jan 24;34(2):543-54
2. A p21/WAF1 mutation favors the appearance of drug resistance to paclitaxel in human noncancerous epithelial mammary cells.
Int J Cancer 2006 Jan 23;
3. Stability of p21(Waf1/Cip1) CDK inhibitor protein is responsive to RhoA-mediated regulation of the actin cytoskeleton.
Oncogene 2006 Jan 16;
4. [Prognostic significance of p21(WAF1) expression in osteosarcoma]
Zhonghua Bing Li Xue Za Zhi 2005 Aug;34(8):524-7
5. Clostridium difficile toxin A-induced colonocyte apoptosis involves p53-dependent p21(WAF1/CIP1) induction via p38 mitogen-activated protein kinase.
Gastroenterology 2005 Dec;129(6):1875-88
6. Oxidative stress response results in increased p21(WAF1/CIP1) degradation in cystic fibrosis lung epithelial cells.
Free Radic Biol Med 2006 Jan 1;40(1):75-86

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中華民國95年06月06日更新