Source:
Sf9 insect cells.
Catalog No.
PRO-388
Since the detection of β2 Glycoprotein I as a target of antiphospholipid antibodies in 1990 the discussion about the clinical relevance of beta2 GP I is still controversial. It is well established that anticardiolipin antibodies are a very heterogeneous group and that beta 2 GP I is only one of several potential targets for anticardiolipin antibodies. Nevertheless it is generally accepted that the binding of β2 GP I to anionic lipids or surface is essential for the differential detection of anticardiolipin antibodies associated with autoimmune diseases (versus those associated with infectious diseases like syphilis, tuberculosis, AIDS, etc).
The close relationship of beta2-GP1 antibodies and cardiolipin antibodies may indicate that the b2 GP I isotypes have the same clinical relevance as the cardiolipin isotypes, where IgM, IgG and IgA autoantibodies have been observed and are routinely determined diagnostic targets. b2GP-1 antibodies and anticardiolipin antibodies can be detected in patients with arterial and venous thrombosis, recurrent fetal loss, thrombosis and SLE. The titers of anticardiolipin and anti-beta2 GP I antibodies usually fluctuate sufficiently that they cannot be used for disease monitoring.
Description : Recombinant Human Apolipoprotein H produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 38,200 dalton. Recombinant Apolipoprotein H is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Physical Appearance: Sterile Filtered clear solution.
Formulation: Recombinant Human beta 2GP-1 is supplied at a of 0.9mg/ml in 16mM HEPES buffer pH-7.6, 200mM sodium chloride, and 20% glycerol.
Stability:
Store at 4蚓 if entire vial will be used within 2-4 weeks. Store, frozen at -20蚓 for longer periods of time.
Please avoid freeze-thaw cycles.
Purity: Greater than 90% (SDS-PAGE). Immunological functions: 1. Binds IgA & IgG-type human auto-antibodies, Auto-antibodies to β2 glycoprotein I recognize conformation dependent and linear epitopes.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including a reference calibrator).
Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody and Goodpasture patient sera.
Coating concentration: 0.5-1 ug/ml (depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination.
Usage: This material is offered by ProSpec-TechnoGene for research, laboratory or further evaluation purposes.
Gene:
Name:APOH
Synonyms:B2G1
Beta-2-glycoprotein I precursor (Apolipoprotein H)
(Apo-H)
(B2GPI)
(Beta(2)GPI)
(Activated protein C-binding protein)
(APC inhibitor)
(Anticardiolipin cofactor).
Domains:
•IPR000436 Sushi/SCR/CCP
